Roar's injury woes are fading at a crucial time for Aloisi, with three key players set to return for the "final" against Perth Glory.
Brett Holman, Corey Brown and Corey Gameiro are all returning from various injuries, meaning for the first time since the beginning of the season, Brisbane can field a near full-strength squad.
Jade North, Michael Theo, Joe Caletti and Nick D'Agostino are the only Roar players not availabale, and all but Caletti should return soon.
Brett Holman, in particular, could be key for Roar's chances. The midfielder is Brisbane's marquee this season, but has only started five matches, leaving a gaping hole in John Aloisi's attacking set up.
Aloisi is recognising the seriousness of his situation, saying every match between now and the end of the season was a "final" for the Roar.
After finishing third in the past two seasons, Brisbane are only two points above Wellington in ninth position, with only three wins this campaign.
Brisbane's Football Director, Craig Moore, also left the club this week due to disagreements with Brisbane's controversial owners, The Bakrie Group, continuing a season of behind-the-scenes turmoil for the Queensland club.
Yet there are still glimmers of optimism in Aloisi's sentiment.
"We need to look forward at the positives and Brett today was brilliant in training," Aloisi said.
"He looks sharp, ready to go, he's hungry. That's only a good sign for us."